DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification

Table 2 Mitochondrial and nuclear quantitative PCR (qPCR) and STR typing result

Sample ID	Mitochondrial DNA	Nuclear DNA
	qPCR	qPCR	qPCR	STR typing (Profiler Plus kit, 10 loci) 34 cycles
	77 bp target (copies/μL)	67 bp target (copies/μL)	67 bp target (ng/μL)
				Number of loci	Percentage genotyping success	None, partial or full profile obtained
4464B	15,727	<1 copy (0.54)	0.001794	8	80%	Partial
10730A	62,592	0	0	Amel only	10%	Partial
8727C	350	<1 copy (0.025)	0.000082	3	30%	Partial
11995A	2,715	<1 copy (0.0091)	0.00003	2	20%	Partial
9210A	n/a	n/a	n/a	n/a	n/a	n/a
Negative control (H20)	0	<1 copy (0.034)	0.000111	0	0%	None
Female positive control	7,896,895	543	1.79213	10	100%	Full

n/a, non-applicable (The sample was excluded from testing due to the low quantity of DNA extract. Quantitative PCR data is presented as a geometric average using triplicate values.).

ISSN: 2041-2223