Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations

Table 3 Primers used in multi-locus sequence typing of Pseduomonas syringae pathovar tomato DC3000

Primer^a	Direction	Sequence (5′ → 3′)
acn-Fp^b	Forward	ACATCCCGCTGCACGCYCTGGCC
acn-Rp^b	Reverse	GTGGTGTCCTGGGAACCGACGGTG
acn-Fs^b	Forward	ATGAARCAGATMGAAGAAATGCGCGG
acn-Rs^b	Reverse	GCCRACCATCTTYTGCGCMAGGG
cts-Fp^b	Forward	AGTTGATCATCGAGGGCGCWGCC
cts-Rp^b	Reverse	TGATCGGTTTGATCTCGCACGG
cts-Fs^b	Forward	CCCGTCGAGCTGCCAATWCTGA
cts-Rs^b	Reverse	ATCTCGCACGGSGTRTTGAACATC
gapA-Fps^b	Forward	CGCCATYCGCAACCCG
gapA-Rps^b	Reverse	CCCAYTCGTTGTCGTACCA
gyrB-Fps^c	Forward	MGGCGGYAAGTTCGATGACAAYTC
gyrB-Rps^c	Reverse	TRATVKCAGTCARACCTTCRCGSGC
pfk-Fp^b	Forward	ACCMTGAACCCKGCGCTGGA
pfk-Rp^b	Reverse	ATRCCGAAVCCGAHCTGGGT
pfk-Fs^b	Forward	AGCAAYATCAAGMTGGCCGA
pfk-Rs^b	Reverse	ACCATGCCKGCCARMAGCG
pgi-Fp^b	Forward	TCAAGGACTTCAGCATGCGCGAAGC
pgi-Rp^b	Reverse	CGAGCCGCCCTGSGCCAGGTACCAG
pgi-Fs^b	Forward	TTCAGCATGCGCGAAGCG
pgi-Rs^b	Reverse	TGCGCCAAGGTACCAGG
rpoD-Fp^c	Forward	AAGGCGARATCGAAATCGCCAAGCG
rpoD-Rps^c	Reverse	GGAACWKGCGCAGGAAGTCGGCACG
rpoD-Fs^b	Forward	AAGCGAATCGAAGAAGGCATYCGTG

ISSN: 2041-2223